For sensitive and high-throughput phosphate determination.

• Very stable reagent
• High sensitivity and wide detection range (0.4 - 50 μM phosphate)
• Can be readily automated on HTS liquid handling systems

The PiBlue™ Phosphate Assay Kit is based on a proprietary formulation of the malachite green dye. The PiBlue™ reagent forms a blue colored complex with free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged. Shelf Life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes or multi-well plates. The assays can be conveniently executed in 96-well plates for high-throughput screening of enzyme inhibitors.