For quantitative determination of xanthine and drug effects on its metabolism.

• Linear detection range 0.01 - 2.5 mM xanthine for colorimetric assays and 3 - 250 µM for fluorimetric assays
• Fast and high-throughput. Can be automated to test thousands of samples per day

Xanthine is a purine base that can be found in most animal tissues and fluids

BioAssay Systems’ xanthine assay kit uses a single Working Reagent that combines the xanthine oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to xanthine concentration in the sample.

It is a product in the purine degradation pathway, produced by guanine deaminase from guanine, and by xanthine oxidoreductase from hypoxanthine. Xanthine is degraded to uric acid by xanthine oxidase. Clinically, xanthine and its derivatives act on sleep-inducing adenosine receptors as antagonists. Simple, direct and high-throughput assays for measuring xanthine find wide applications in research and drug discovery.