For quantitative determination of superoxide dismutase (SOD) enzyme activity.

• Linear detection range of 0.05 - 3 U/mL SOD
• No wash and reagent transfer steps are involved
• Homogeneous "mix-incubate-measure" type high-throughput assay

Superoxide dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide into O2 and H2O2

BioAssay Systems’ SOD assay provides a convenient colorimetric means for the quantitative determination of SOD enzyme activity in biological samples. In the assay, superoxide (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a WST-1 dye to form a colored product. SOD scavenges the O2- thus less O2- is available for the chromogenic reaction. The color intensity (OD440nm) is used to determine the SOD activity in a sample.

They are an important antioxidant defense in all cells exposed to O2. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders and cancer.