For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.

• Linear detection range 2 - 500 μM pyruvate for colorimetric assays and 0.2 - 50 μM for fluorimetric assays
• Convenient room temperature, high-throughput assay

Pyruvate is a key intermediate in cellular metabolic pathways

BioAssay Systems’ pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to pyruvate concentration in the sample.

Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready. Procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.