For rapid, quantitative determination of monoamine oxidase activity.

• Safe
• Non-radioactive assay
• As low as 0.01 U/L MAO activity can be quantified.
• Can be readily automated to test thousands of samples per day

Monoamine oxidases (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines

BioAssay Systems’ MAO Assay Kit provides a convenient fluorimetric means to measure MAO enzyme activity. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorimetric method (λex/em = 530/585nm). The assay is simple, sensitive, stable and high-throughput adaptable.

MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAOs in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression.