Specifications
- Description:Fumarase Assay Kit
- Size:100 assays
- Method:OD565 nm
- Sample:Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media).
- Processing Time:40 minutes
- Cat. No.:75878-090
- Supplier no.:EFMR-100
Specifications
About this item
For quantitative determination of fumarase enzyme activity.
- Linear detection range 0.4 to 70 U/L
- Homogeneous "mix-incubate-measure" type high-throughput assay
Fumarase (or fumarate hydratase) (EC 4.2.1.2) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate
BioAssay Systems’ non-radioactive, colorimetric fumarase assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc.