For quantitative fluorimetric determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.

• Linear detection range 1 - 50 μM L-lactate in 96-well plate assay
• Can be readily automated to test thousands of samples per day

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions

BioAssay Systems’ EnzyFluo™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the lactate concentration in the sample.

Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready. Procedures for measuring lactate concentration are very desirable.