For quantitative determination of creatine and evaluation of drug effects on creatine metabolism.

• Linear detection range 4 to 1000 μM (colorimetric assay) or 0.5 to 50 μM (fluorimetric assay)
• Simple "mix-and-measure" procedure allows reliable quantitation of creatine within 30 minutes

Creatinine is present in vertebrates and helps to supply energy to muscle

BioAssay Systems’ creatine assay is based on enzymatic reactions leading to formation of a pink colored product. The optical density at 570 nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to the creatine concentration in the sample.

In humans and animals, approximately half of creatine originates from food (mainly from fresh meat). Creatine supplementation has been investigated as a possible therapeutic approach for the treatment of muscular, neuromuscular, neurological and neurodegenerative diseases. Simple, direct and automation-ready. Procedures for measuring creatine are popular in research and drug discovery.