For quantitative determination of alpha-glucosidase activity.

• Linear detection range 2 to 250 U/L
• Simple "mix-and-measure" procedure allows reliable quantitation of α-glucosidase activity within 20 minutes
• All reagents are compatible with high-throughput liquid handling instruments

α-Glucosidase hydrolyzes the terminal, non-reducing 1,4-linked α-D-glucose residues with release of α-D-glucose

α-Glucosidase Assay Kit is designed to measure α-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-α-D-glucopyranoside that is hydrolyzed specifically by α-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

α-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready. Procedures for measuring α-glucosidase activity are becoming popular in Research and Drug Discovery. BioAssay Systems’ QuantiChrom™.