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Specifications
- Antibody Type:Primary
- Antigen Name:CD45
- Antigen Symbol:CD45
- Clonality:Monoclonal
- Clone:LT40
- Conjugation:Spectral Red® RPE (R-Phycoerythrin)/Cy5®
- Flow Cytometry:Yes
- Host:Mouse
- Isotype:IgM
- Reactivity:Chicken
- Size:0.1 mg
- Form:Liquid
- Gene ID:386580
- Storage Buffer:Supplied in PBS/NaN3 and a stabilizing agent
- Storage Temperature:2-8°C
- Concentration:0.1 mg/mL
- Shipping Temperature:Ambient Temperature
- Immunogen:Chicken bursal lymphocytes
- Cat. No.:100245-486
- Supplier no.:8270-13
Specifications
About this item
Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45. Levels of CD45 expression detected by the monoclonal antibody LT40 in the avian thymus are heterogeneous with approximately 90% of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10% (CD45low) of thymocytes. The CD45low population contains exclusively thymocytes with the CD3-CD4-CD8low phenotype characteristic of the immediate precursors to the CD3-CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8low to CD4+CD8+ and before the expression of CD3. The ability of the monoclonal antibody LT40 to discriminate this CD45low subpopulation provides a suitable marker for the CD3-CD4-CD8low immediate precursors to the CD3-CD4+CD8+ thymocytes.
Type: Primary
Antigen: CD45
Clonality: Monoclonal
Clone: LT40
Conjugation: Spectral Red® RPE (R-Phycoerythrin)/Cy5®
Epitope:
Host: Mouse
Isotype: IgM
Reactivity: Chicken