PureYield Plasmid Midiprep System provides a fast method for isolating transfection-quality plasmid DNA. Yields 100-200ug of plasmid DNA from 50ml bacteria culture.

• Up to 200µg of Transfection-Ready Plasmid DNA from 50ml Cultures
• 30-minute, vacuum-based protocol
• Highly pure, concentrated plasmid DNA
• No tedious, gravity-drip columns or post-elution alcohol precipitation

The PureYield Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA. The system provides a rapid method for purification of 100-200ug of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400ug of high-copy-number plasmid from 250ml of E. coli culture. The PureYield Plasmid Midiprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man Laboratory Vacuum Manifold).

Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in-vitro transcription, and coupled in-vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation to provide rapid purification from a single method. The system has been designed for use with centrifugation or vacuum.

Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, which reduces the time spent on purification. An alternative protocol allows purification of over 400 μg of high-copy-number plasmid from 250 mL of E. coli culture. It incorporates a unique Endotoxin Removal Wash that's designed to remove substantial amounts of protein, RNA, and endotoxin contaminants from purified plasmid DNA.