A highly sensitive, flluorescent alkaline phosphatase substrate.

The AttoPhos® Substrate (2´-[2-benzothiazoyl]-6´-hydroxybenzothiazole phosphate [BBTP]) is cleaved by alkaline phosphatase (AP) to produce inorganic phosphate (Pi) and the alcohol, 2´-[2- benzothiazoyl]-6´- hydroxybenzothiazole (BBT). This enzyme-catalyzed conversion of AttoPhos® Substrate to BBT is accompanied by an enhancement in fluorescence properties that include increased quantum efficiency plus fluorescence excitation and emission maxima that are red-shifted further into the visible region.

The AttoPhos® Buffer is optimized for sensitive detection of alkaline phosphatase (AP) when used to prepare working solutions with AttoPhos® Substrate