TMB One Solution is a chromagen substrate, 3,3',5,5'-tetramethylbenzidine (TMB) provided in a mildly acidic, nonhazardous buffer for HRP detection in ELISA format.

• Substrate for Horseradish Peroxidase Detection in ELISAs
• Single solution provided ready-to-use; just add, incubate, stop and read
• Low background provides greater assay sensitivity

The substrate is provided as a single solution at a ready-to-use working dilution.

It develops a blue reaction product when oxidized by peroxidase and a yellow reaction product in an endpoint multiwell assay after the addition of an acid solution provided by the end user.

This product is not recommended for Western analysis or immunohistochemistry.