Middlebrook 7H9 Broth dehydrated base supplemented with glycerol supports the growth of mycobacteria, including M

• Inorganic salts provides essential growth substances
• Sodium citrate holds certain inorganic cations in solution
• Glycerol provides carbon and energy
• Malachite green dye partially inhibits bacterial contaminants

Middlebrook 7H9 Broth with appropriate supplements is primarily used for growth of pure cultures of mycobacteria for use in laboratory studies.

tuberculosis.