Kligler Iron Agar aids in the differentiation of gram-negative enteric bacilli on the basis of their ability to ferment dextrose and lactose and produce sulfides.

• Casein and animal tissue provide essential nutrients
• Hydrogen sulfide production is detected by ferric ammonium citrate and sodium thiosulfate
• Black color formation is evidence of hydrogen sulfide production
• Gas production is detected as individual bubbles or displacement of agar

Kligler Iron Agar is used for the differentiation of members of the Enterobacteriaceae on the basis of their ability to ferment dextrose and lactose and to produce sulfides.