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T4 DNA Ligase 10X Buffer, Promega
T4 DNA Ligase 10X Buffer, Promega
Catalog # PAC1263
CAS Number:  
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T4 DNA Ligase 10X Buffer, Promega
Catalog # PAC1263
Supplier Number:  C1263
CAS Number:  
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Specifications

  • Application:
  • Description:
    T4 DNA Ligase 10X Buffer
  • Size:
    3×500 µl
  • Environmentally Preferable:
  • Cat. No.:
    PAC1263
  • Supplier no.:
    C1263

Specifications

About this item

For ligation of DNA ends.

  • Joins Two DNA Strands with Cohesive or Blunt Ends
  • Use with 5´, 3´ or blunt-ended dsDNA (e.g., inserts and vectors)
  • Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
  • Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule

T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids.