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Specifications
- Description:T4 DNA Ligase 10X Buffer
- Size:3×500 µl
- Cat. No.:PAC1263
- Supplier no.:C1263
Specifications
About this item
For ligation of DNA ends.
- Joins Two DNA Strands with Cohesive or Blunt Ends
- Use with 5´, 3´ or blunt-ended dsDNA (e.g., inserts and vectors)
- Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
- Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule
T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids.