About this item
The innuPREP RNA Mini Kit is a jack-of-all-trades when it comes to extracting total RNA that is excellent in terms of both quality and quantity.
- Fast, efficient purification of total RNA from a wide variety of different starting materials and in varying amounts
- Prefiltration to selectively remove genomic DNA with no DNase digestion
- Eliminates the need for using highly toxic β-mercaptoethanol
- Ready-to-use RNA isolated after only 15-40 minutes; quality and quantity of isolated RNA is excellent
The innuPREP RNA Mini Kit is a jack-of-all-trades when it comes to extracting total RNA that is excellent in terms of both quality and quantity. The specially optimized lysis buffer system guarantees isolation of intact RNA and lasting deactivation of endogenous and exogenous RNases. A pre-column contained in the kit can be used to remove genomic DNA, which virtually eliminates the traditional need for DNase I digestion. The RNA is then bound to a second spin filter membrane, washed and finally eluted in 30-80µl of RNase-free water. As such, the extraction process can be concluded in 15-40 minutes, depending on the starting material.
Specifications
Starting material: Eukaryotic cells (max. 5 × 10^6); Tissue samples (max. 20mg); Gram+ and gram– bacteria (max. 1 × 10^9); Biopsie
Column binding capacity: approx. 100µg RNA
Average purity: 1.8 – 2.1
Extraction time: Approx. 15 - 40 minutes
Average yield: Up to 100µg RNA, depending on the type and quantity of starting material
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* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:Total RNA
- Sample type:BacteriaCultured cellsHuman/animal cells and tissues
- Target molecule:RNA
- Description:innuPREP RNA Mini Kit
- Yield:Up to 100µg RNA, depending on the type and quantity of starting material