About this item
The innuPREP DOUBLEpure Kit allows efficiently extracting of DNA fragments from TAE or TBE agarose gels, and utilizes a novel 2-step technology for purifying amplification products from PCR reaction mixtures.
- Combination kit for fast extraction of DNA fragments from agarose gels, or amplification products from PCR reaction mixtures
- Flexible elution volumes between 30 and 50 µl (standard protocol) and 10 to 20 µl (“Mini-Elute” protocol)
- High recovery rates of up to 95%
- Capable of processing fragment lengths of up to 30 kb
The innuPREP DOUBLEpure Kit allows efficiently extracting of DNA fragments from TAE or TBE agarose gels, and utilizes a novel 2-step technology for purifying amplification products from PCR reaction mixtures. The spin filter column has a binding capacity of over 20 µg, making it possible to achieve high yields and excellent quality when isolating DNA fragments. In addition, the kit also produces recovery rates of up to 95% depending on the fragment size. Other outstanding features of the innuPREP DOUBLEpure Kit include easy handling, flexible settings for elution volumes, and fast purification protocols.
Specifications
- Fragment length:PCR purification: > 60 bp – 30 kb
Gel extraction: 100 bp – 30 kb - Recovery:PCR purification: approx. 60 % to 95 %
Gel extraction: approx. 60 % to 90 %
Depends on fragment length - Binding Capacity:Column binding capacity: > 20 µg DNA
- Contents:PCR reaction mixes (up to 50 µl)
TAE agarose gels (up to 300 mg)
TBE agarose gels (up to 300 mg) - Time:PCR purification: approx. 3 minutes (2-step process)
Gel extraction: approx. 20 minutes