APT Agar is used for cultivating heterofermentative lactobacilli and other organisms requiring high thiamine content.

• Peptone is a source of carbon, nitrogen, vitamins, and minerals
• Yeast extract supplies B-complex vitamins to stimulate growth
• Dextrose is the carbohydrate
• Polysorbate 80 is a source of fatty acids
• Agar is the solidifying agent in APT Agar

The manganese chloride, magnesium sulfate, and ferrous sulfate provide ions used in replication by lactobacilli.

APT Broth is used for culturing Weissella viridescens ATCC 12706 used in the assay of thiamine. It is also used for cultivating heterofermentative lactobacilli and other organisms requiring high thiamine content.