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Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Direct
- Conjugate ELISA:HRP
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:RF-IgM, IgM rheumatoid factor
- Description:IgM rheumatoid factor mouse ELISA
- Size:1 kit
- Sample Type:Serum, plasma-EDTA, plasma-citrate
- Detection Method:Colorimetric
- Shelf Life:See expiration date
- Detection Range:15.6 - 1 000 mU/ml
- Storage Temperature:2…8 °C
- Sample Volume:1 µl
- Sensitivity:0.78 mU/ml
- Regulatory Status:RUO
- Cat. No.:101424-362
- Supplier no.:RSHAKRRG111R
Specifications
About this item
96 wells (1 kit) - ELISA is a competitive enzyme immunoassay for the quantitative measurement of IgM Rheumatoid Factor.
- Calibration Range: 15.6–1000 mU/ml
- Limit of Detection: 0.78 mU/ml
- Plasma-Citrate, Plasma-EDTA, Serum: 1 ul/well
Mouse IgM Rheumatoid factor ELISA Kit is a sandwich ELISA system for quantitative measurement of mouse IgM Rheumatoid factor antibody titer.
Rheumatoid factors are autoantibodies against Fc region of of IgG and is found in 70-80% of patients suffering from chronic rheumatoid arthritis, and are considered to be closely related to its pathological symdrome. Experimental animal models with spontaneous autoimmune diseases similar to those in humans, and animals with artificially-induced inflammation have been used to elucidate the mechanism of autoimmune diseases and to search for potential new remedies. A representative model animal of spontaneous autoimmune diseases is MRL/lpr mouse. As MRL/lpr shows high incidence of lymph node tumor, nephritis, angitis, and arthritis, this animal strain is useful for studies of the mechanism of human autoimmune diseases including rheumatoid arthritis. Autoantibodies found in MRL/lpr serum are IgG type rheumatoid factor, IgM type rheumatoid factor, anti-ssDNA antibodies, anti-dsDNA antibodies, and anti-Sm antibody, etc. This kit enables quantification and comparison of IgM type rheumatoid factor with a calibration curve using standard antibody preparation.
In Shibayagi’s Mouse IgM Rheumatoid factor ELISA Kit, standards or samples are incubated in monoclonal mouse rheumatoid factor antigen-coated wells to capture IgM Rheumatoid factor. After 2 hours incubation and washing, HRP (horse radish peroxidase)-labeled anti-mouse IgM is added and incubated for 2 hours together with captured anti-mouse IgM. After washing, HRP-complex remaining in wells is reacted with a chromogenic substrate (TMB) for 20 minutes, and reaction is stopped by addition of acidic solution, and absorbance of yellow product is measured spectrophotometrically at 450 nm. The absorbance is nearly proportional to IgM Rheumatoid factor concentration. The standard curve is prepared by plotting absorbance against standard IgM Rheumatoid factor concentrations. IgM Rheumatoid bfactor concentrations in unknown samples are determined using this standard curve.