Lactobacilli MRS Broth is recommended for the isolation, enumeration and cultivation of Lactobacillus spp.

• Clear, with a very slight opalescence and dark amber in color
• Provide luxuriant growth of lactobacilli isolated from oral, fecal, and other specimen sources
• Supports good growth and useful for a number of fastidious strains
• Used as a basal medium for fermentation tests

MRS Broth was developed by deMan, et al., to provide luxuriant growth of lactobacilli isolated from oral, fecal, and other specimen sources

Peptone and dextrose provide the carbon, nitrogen and other elements necessary for growth. Tween ® 80, acetate, magnesium and manganese provide growth factors needed for culturing a variety of lactobacilli. Those ingredients may also inhibit the growth of some organisms other than lactobacilli. In addition, tomato juice is not required. In a slightly modified form, it can also be used as a basal medium for fermentation tests.

(6) Lactobacilli MRS Broth is an improved medium for lactobacilli, it supports good growth and is particularly useful for a number of fastidious strains which grow poorly on other general media.