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Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:IgE
- Description:Immunoglobulin E mouse ELISA
- Size:1 kit
- Sample Type:Serum, plasma
- Detection Method:Colorimetric
- Shelf Life:See expiration date
- Detection Range:1 - 100 ng/ml
- Storage Temperature:2…8 °C
- Sensitivity:50 pg/ml
- Regulatory Status:RUO
- Cat. No.:101424-350
- Supplier no.:RSHAKRIE010R
Specifications
About this item
96 wells (1 kit) - ELISA is a competitive enzyme immunoassay for the quantitative measurement of IgE.
- Calibration Range: 1-100 ng/ml
- Limit of Detection: 50 pg/ml
- Plasma, Serum: 5 ul/well
LBIS Mouse IgE ELISA Kit is a sandwich ELISA system for quantitative measurement of mouse IgE.
Heave chains (Hε). In electrophoresis, it moves to γ1 region. Its biological half life is about 3 days, and its blood level in normal human subject is very low, about 300ng/mL. The blood level of IgE increases markedly in parasite infection and in hay fever. IgE that is responsible for allergy has been called “reagin”. Sensitization by an allergen increases reagin IgE which binds to FcεR1 receptor in basophilic leucocytes and mast cells at Fc region and sensitizes those cells. If the allergen binds the sensitized cells, those cells will be degranulated and release histamin, serotonin, protease, heparin, chemotactic factor, prostaglandins, leucotriens, and so on, causing bronchoconstriction, mucous edema, and hypersecretion, and leads to type I allergic reactions like bronchial asthma, hives, allergic rhinitis, anaphylaxis, and so on. This is the assay kit for total mouse IgE. Shibayagi is also providing assay kits for allergen (OVA) -specific mouse IgE.
In Shibayagi’s LBIS Mouse IgE ELISA Kit, standards or samples are incubated in monoclonal antibody coated wells to capture IgE. After 2 hours’ incubation and washing, biotinylated anti-mouse IgE antibody is added and incubated further for 2 hours’ to bind with captured IgE. After washing, HRP (horse radish peroxidase)-conjugated streptavidin is added, and incubated for 1 hour. After washing, bound HRPconjugated streptavidin is reacted with a chromogen (TMB) for 20 minutes, and reaction is stopped by addition of acidic solution, and absorbance of yellow product is measured spectrophotometrically at 450 nm. The absorbance is nearly proportional to IgE concentration. The standard curve is prepared by plotting absorbance against standard IgE concentrations. IgE concentrations in unknown samples are determined using this standard curve.