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Specifications
- Primary antibody reactivity:Human
- Description:Immunoglobulin Free Light Chains Kappa and Lambda Human ELISA
- Size:1 kit
- Regulatory Status:RUO
- Cat. No.:200068-238
- Supplier no.:RD194088100
Specifications
About this item
In serum, FLC kappa exists predominantly as a monomer with a molecular weight of 22.5 kDa and FLC lambda as a dimer with a molecular weight of 45 kDa.
- Calibration Range: 10–320 ug/L (FLC kappa) 17.5–560 ug/L (FLC lambda)
- Limit of Detection: 0.6 ug/L
- Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum, Urine: 2x5 uL/well
Human immunoglobulin molecules consist of two identical heavy chains which define immunoglobulin classes (IgG, IgA, IgM, IgD and IgE) and identical light chains (kappa or lambda) that are covalently linked to a heavy chain. In healthy individuals, the majority of light chains in serum exist bound to heavy chain. However, low levels of free light chains (FLCs) are found in serum of normal individuals due to their excess production over heavy chains by mature B-cells. In serum, FLC kappa exists predominantly as a monomer with a molecular weight of 22.5 kDa and FLC lambda as a dimer with a molecular weight of 45 kDa. This size difference results in a differential glomerular filtration rate and, consequently, a ratio of FLC kappa to FLC lambda of 1:1.6 in serum. FLCs are observed in urine too but filtration and reabsorption of low molecular proteins in the kidney strongly affects the FLC concentration so that urinary FLC level is low in healthy individuals.