Specifications
- Protein/Peptide Type:Recombinant
- Source:Insect cells (Sf21)
- Species:Human
- Size:10 μg
- Tag sequence:His Tag
- Storage Conditions:Stable for at least 6 months after receipt when stored at −80 °C.
- Biological Activity:0.5% of wild type PARP-1.
- Protein Synonyms:ARTD1
- Protein/Peptide Name:PARP1 E988K Mutant Control
- Purity:>95% (SDS-PAGE)
- Formulation:Liquid, In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol.
- Cat. No.:75794-152
- Supplier no.:90-343
Specifications
About this item
PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E998K mutant) is an inactive form of PARP-1 which can be used as a control compound.
- Fusion-Tag: His Tag
This recombinant proteins is for research use only.