Specifications
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Size:10 μg
- Tag sequence:isoleucine zipper motif Tag
- Storage Conditions:Stable for at least 6 months after receipt when stored at −20 °C.
- Biological Activity:Induces apoptosis in vitro in different human cancer cell lines with an EC50 of 5-200ng/ml, depending on the individual cell line used. Recombinant izTRAIL does not kill 4-day cultures of primary human hepatocytes (PHH) at concentrations of at least up to 1ug/ml (Ganten 2006). Good bioavailability in vivo, shows no toxic effects in mice at doses of at least up to 500ug per day (Wissink 2006).
- Protein Synonyms:Apo-2L|CD253|TNFSF10
- Protein/Peptide Name:IZTRAIL (soluble)
- Purity:>95% (SDS-PAGE)
- Sequence:95-281
- Formulation:Lyophilized, Contains 20mM TRIS-Cl, 0.5M arginine-HCl, 100mM NaCl, 0.02% Tween 20.
- Cat. No.:75794-020
- Supplier no.:90-257
Specifications
About this item
izTRAIL is a newly available, highly active recombinant form of soluble human TRAIL. Due to a trimerizing N-terminal isoleucine zipper (iz) motif the intrinsic trimerization of TRAIL, required for apoptosis-inducing activity of TRAIL, is enhanced when compared to non-tagged soluble human TRAIL (shTRAIL). Therefore, izTRAIL is a potent inducer of apoptosis in many human cancer cells, but not normal human hepatocytes. In addition, the half-life of izTRAIL is about eight-fold higher than the half-life of shTRAIL. These properties render izTRAIL highly suitable for both, in vitro and in vivo use, particularly for studies in which investigators plan to transfer their in vitro results into an in vivo system with human cancer cells in xenotransplant settings examining susceptibility to TRAIL-induced apoptosis.
- Fusion-Tag: isoleucine zipper motif Tag
This recombinant proteins is for research use only.