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E. coli Recombinant RNA pyrophosphohydrolase (from E. coli)
Catalog # 75791-798
Supplier:  Prosci
CAS Number:  
E. coli Recombinant RNA pyrophosphohydrolase (from E. coli)
Catalog # 75791-798
Supplier:  Prosci
Supplier Number:  92-618
CAS Number:  

Specifications

  • Enzyme type:
    Recombinant
  • Source:
    E. coli
  • Species:
    E. coli
  • Size:
    0.05 mg
  • Storage Conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Endotoxin Content:
    <0.1 ng/ug (1 IEU/ug) as determined by LAL test.
  • Enzyme Name:
    RNA pyrophosphohydrolase
  • Enzyme Synonyms:
    RNA pyrophosphohydrolase, (Di)nucleoside polyphosphate hydrolase, Ap5A pyrophosphatase, rppH
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular Weight:
    20.8 kD
  • Sequence:
    Met1-Gly176
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 50 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH₂O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Cat. No.:
    75791-798
  • Supplier no.:
    92-618

Specifications

About this item

Messenger RNA (mRNA) degradation plays a key role in the control of gene expression in all organisms by limiting the number of times that each mRNA molecule can be used as a template for protein synthesis

This recombinant protein can be used for biological assays. For research use only.

RNA pyrophosphohydrolase, also called RppH, is a master regulator of 5'-dependent mRNA decay. It accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage. RppH preferentially hydrolyzes diadenosine penta-phosphate with ATP as one of the reaction products, and can be able to hydrolyze diadenosine hexa- and tetra-phosphate. However, this protein has no activity on diadenosine tri-phosphate, ADP-ribose, NADH and UDP-glucose. In the meningitis causing strain E. coli K1, it has been shown to play a role in HBMEC (human brain microvascular endothelial cells) invasion in vitro.