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Specifications
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Size:0.05 mg
- Environmentally Preferable:
- Tag sequence:N-6 His tag
- Storage Conditions:Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
- Endotoxin Content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Protein Synonyms:S-100 protein beta chain|S100 calcium-binding protein B|S100 calcium binding protein B|S-100 protein subunit beta|Protein S100-B|S100 beta|S100b
- Protein/Peptide Name:S100 calcium binding B
- Purity:> 95% as determined by reducing SDS-PAGE
- Molecular Weight:12.2 kD
- Sequence:Met1-Glu92
- Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PBS,150mM NaCl,pH7.4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Cat. No.:75791-784
- Supplier no.:92-611
Specifications
About this item
S100-B, is an acidic protein with a molecular weight of 21 kDa belonging to the S100 family. S100-B contains two EF-hand-type calcium-binding motifs separated by a hinge region with a hydrophobic cleft. S100-B plays an important role in neurodevelopment, differentiation, and brain construction. S100-B has neuroprotective effects, but at high concentrations S100-B is neurotoxic. Extracellular concentration of S100-B increases following brain damage, which easily penetrates into cerebrospinal fluid in brain damage and then into the blood. S100-B is expressed and produced by astrocytes in vertebrate brains and in the CNS, and the astrocytes are the major cells producing S100-B protein in gray matter, as well as oligodendrocytes are the predominant S100-B in protein producing cells in white matter. The major advantage of using S100-B is that elevations in serum or CSF levels provide a sensitive measure for determining CNS injury at the molecular level before gross changes develop, enabling timely delivery of crucial medical intervention before irreversible damage occurs. In addition, S100-B, which is also present in human melanocytes, is a reliable marker for melanoma malignancy both in bioptic tissue and in serum.
- Fusion-Tag: N-6 His tag
This recombinant protein can be used for biological assays. For research use only.