Specifications
- Enzyme type:Recombinant
- Source:E. coli
- Species:Human
- Size:0.05 mg
- Tag sequence:C-6 His tag
- Storage Conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Endotoxin Content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Enzyme Name:beta-1,3-Glucuronyltransferase 3
- Enzyme Synonyms:B3GAT3, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3, Beta-1, 3-glucuronyltransferase 3, Glucuronosyltransferase I, GlcAT-I, GlcUAT-I, Gal beta-1, 3-Gal-R glucuronyltransferase, REF: C1005
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular Weight:30.4 kD
- Sequence:Glu72-Val335
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM Tris, 150 mM NaC,2 mM EDTA, 20% glycerol,pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Cat. No.:75791-574
- Supplier no.:92-502
Specifications
About this item
Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3) is an enzyme that in humans is encoded by the B3GAT3 gene, belongs to the glycosyltransferase 43 family
This recombinant protein can be used for biological assays. For research use only.
Fusion-Tag: C-6 His tag
B3GAT3 is involved in a number of biological processes such as catalyzing the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans, forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate, gGlycosaminoglycans biosynthesis, transfering a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans.It also plays a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins , hows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc and stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation.