Specifications
- Protein/Peptide Type:Recombinant
- Species:Mouse
- Size:0.05 mg
- Tag sequence:C-Fc tag
- Storage Conditions:Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
- Endotoxin Content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Protein Synonyms:PD-1|mPD-1|CD279|Pdcd1|Programmed Cell Death protein 1
- Protein/Peptide Name:PDCD1
- Purity:> 95% as determined by reducing SDS-PAGE
- Molecular Weight:43.3 kD
- Sequence:Leu25-Gln167
- Formulation:Lyophilized from a 0.2 um filtered solution of 20mM Tris,150mM NaCl,pH8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Cat. No.:75791-560
- Supplier no.:92-495
Specifications
About this item
Programmed Death-1 (PD-1), firstly cloned from mouse T cell hybridoma 2B4.11, is one member of CD28/CTLA-4 superfamily. PD-1 belongs to type I transmembrane protein and acts as an important immunosuppressive molecule. This family also include members of CD28, CTLA-4 and ICOS.The mouse Programmed Death-1 protein, encoded by PD-1 gene, comprises four parts including a putative 20 aa signal peptide, a 149 aa extracellular region, a 21 aa transmembrane domain and a 98 aa cytoplasmic region. The cytoplamsic tail of PD-1 contains two structural motifs, an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM) formed by two tyrosine residues which make the difference in PD-1 signal mediating. Mouse PD-1 is expressed in thymus and shares about 69% aa sequence identity with human PD-1. Recently, programmed death-1 (PD-1) with its ligands, programmed death ligand B7H1 (PD-L1) and B7DC (PD-L2), was found to regulate T-cell activation and tolerance, upon ligand binding, inhibiting T-cell effector functions in an antigen-specific manner. PD-1 gene knocked out mice would induce some autoimmune diseases, which suggests that PD-1 acts as a co-inhibitory molecule actively participating in maintaining peripheral tolerance. Thus, PD-1 may be a useful target for the immunologic therapy of carcinoma,infection,autoimmune diseases as well as organ transplantation.
- Fusion-Tag: C-Fc tag
This recombinant protein can be used for biological assays. For research use only.