Order Entry
Puerto Rico
ContactUsLinkComponent
Human Recombinant Lysine--tRNA Ligase (from Cells)
Human Recombinant Lysine--tRNA Ligase (from Cells)
Catalog # 75789-988
Supplier:  Prosci
CAS Number:  
Human Recombinant Lysine--tRNA Ligase (from Cells)
Catalog # 75789-988
Supplier:  Prosci
Supplier Number:  91-664
CAS Number:  

Specifications

  • Enzyme type:
    Recombinant
  • Source:
    Cells
  • Species:
    Human
  • Size:
    0.05 mg
  • Tag sequence:
    C-6 His tag
  • Storage Conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Endotoxin Content:
    <0.1 ng/ug (1 IEU/ug) as determined by LAL test.
  • Enzyme Name:
    Lysine--tRNA Ligase
  • Enzyme Synonyms:
    Lysine--tRNA Ligase, Lysyl-tRNA Synthetase, LysRS, KARS, KIAA0070
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular Weight:
    69.1 kD
  • Sequence:
    Ala2-Val597
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl,1 mM DTT, 20% glycerol,pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested Applications:
    Bioassay
  • Cat. No.:
    75789-988
  • Supplier no.:
    91-664

Specifications

About this item

Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family

This recombinant protein can be used for biological assays. For research use only.

Fusion-Tag: C-6 His tag

The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).