Specifications
- Enzyme type:Recombinant
- Source:Cells
- Species:Human
- Size:0.05 mg
- Tag sequence:C-6 His tag
- Storage Conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Endotoxin Content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Enzyme Name:Lysine--tRNA Ligase
- Enzyme Synonyms:Lysine--tRNA Ligase, Lysyl-tRNA Synthetase, LysRS, KARS, KIAA0070
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular Weight:69.1 kD
- Sequence:Ala2-Val597
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl,1 mM DTT, 20% glycerol,pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested Applications:Bioassay
- Cat. No.:75789-988
- Supplier no.:91-664
Specifications
About this item
Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family
This recombinant protein can be used for biological assays. For research use only.
Fusion-Tag: C-6 His tag
The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).