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Human Recombinant Carboxypeptidase A1 (from Cells)
Catalog # 75789-732
Supplier:  Prosci
CAS Number:  
Human Recombinant Carboxypeptidase A1 (from Cells)
Catalog # 75789-732
Supplier:  Prosci
Supplier Number:  91-528
CAS Number:  

Specifications

  • Enzyme type:
    Recombinant
  • Source:
    Cells
  • Species:
    Human
  • Size:
    0.05 mg
  • Tag sequence:
    C-6 His tag
  • Storage Conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Endotoxin Content:
    <0.1 ng/ug (1 IEU/ug) as determined by LAL test.
  • Enzyme Name:
    Carboxypeptidase A1
  • Enzyme Synonyms:
    Carboxypeptidase A1, CPA1, CPA
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular Weight:
    46.57 kD
  • Sequence:
    Lys17-Tyr419
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested Applications:
    Bioassay
  • Cat. No.:
    75789-732
  • Supplier no.:
    91-528

Specifications

About this item

Carboxypeptidase A1 (CPA1) is secreted as a pancreatic peptidase that comes from the precursor form of inactive procarboxypeptidase

This recombinant protein can be used for biological assays. For research use only.

Fusion-Tag: C-6 His tag

CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. It has a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 cleaves the C-terminal amide or ester bond of peptides and involves in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E.