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Specifications
- Size:250 U
- Enzyme Name:DNase, Double-Strand Specific, Heat-Labile
- Cat. No.:10147-174
- Supplier no.:1B1662-250U
Specifications
About this item
VWR® Life Science's DNase, Double-Strand Specific, Heat-Labile is a recombinant endonuclease that cleaves phosphodiester bonds in DNA to yield 2 – 8 bp oligonucleotides with 5’-phosphate and 3’-hydroxyl termini
- High specificity for dsDNA, while leaving ssDNA and RNA intact
- Heat inactivates completely and irreversibly at 55°C
- Ideal for genomic DNA removal in RNA preps
- Removes contaminating dsDNA from PCR master mixes
Store frozen (-20 – 0°C). Stable two years when stored frozen (-20 – 0°C) and up to six months when stored cold (2 – 8°C). The enzyme tolerates multiple freeze-thaw cycles.
The high specific activity of this enzyme toward dsDNA can be inactivated by heating at 55°C, conveniently eliminating the need for its physical or chemical removal before downstream processing, even in the presence of RNA and ssDNA, such as primers and probes. DNase, Double-Strand Specific, Heat-Labile is ideal for removal of genomic DNA in RNA preps and for removal of DNA carryover contamination in PCR mixes, before addition of template.