VWR® Life Science's DNase, Double-Strand Specific, Heat-Labile is a recombinant endonuclease that cleaves phosphodiester bonds in DNA to yield 2 – 8 bp oligonucleotides with 5’-phosphate and 3’-hydroxyl termini

• High specificity for dsDNA, while leaving ssDNA and RNA intact
• Heat inactivates completely and irreversibly at 55°C
• Ideal for genomic DNA removal in RNA preps
• Removes contaminating dsDNA from PCR master mixes

Store frozen (-20 – 0°C). Stable two years when stored frozen (-20 – 0°C) and up to six months when stored cold (2 – 8°C). The enzyme tolerates multiple freeze-thaw cycles.

The high specific activity of this enzyme toward dsDNA can be inactivated by heating at 55°C, conveniently eliminating the need for its physical or chemical removal before downstream processing, even in the presence of RNA and ssDNA, such as primers and probes. DNase, Double-Strand Specific, Heat-Labile is ideal for removal of genomic DNA in RNA preps and for removal of DNA carryover contamination in PCR mixes, before addition of template.