Biotinylated probes for detection of His-tagged proteins.

• Detect as little as 0.1 pmol histidine-tagged proteins immobilized on nitrocellulose membranes
• Biotin NTA can be removed from the histidine-tagged protein at pH 4.8, allowing the blot be reanalyzed with another probe
• X linker between biotin and probe tends to facilitate interaction with streptavidin

Biotin NTA, also known as BNTA (Biotin nitrilotriacetic acid, tripotassium salt) reversibly binds to histidine-tagged proteins, and can be detected with Biotium's CF™ Dye streptavidin or monoclonal anti-biotin fluorescent conjugates. Available with 6-atom aminohexanoyl (X) linker between the biotin and NTA to facilitate interaction with streptavidin.