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Specifications
- Antibody Type:Secondary
- Antigen Name:Immunoglobulin G
- Antigen Symbol:IgG F(c) gamma
- Clonality:Polyclonal
- Conjugation:HRP (Horseradish Peroxidase)
- ELISA:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- Isotype:IgG
- Reactivity:Human
- Western Blot:Yes
- Size:1 ml
- Epitope:F(c) gamma
- Cross Adsorption:(min x Bovine, Horse, Mouse Serum Proteins)
- Form:Freeze-dried powder
- Storage Buffer:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15mg/mL BSA
- Storage Temperature:4 °C
- Concentration:0.8 mg/mL
- Shipping Temperature:ambient
- Purification:Affinity Purified
- Cat. No.:100186-432
- Packaging:Glass ampoule
- Supplier no.:209-035-098
Specifications
About this item
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody exhibits inherent minimal cross-reaction to mouse serum proteins and has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins. The antibody may cross-react with immunoglobulins from other species.
- Antibody Format: Whole IgG
Suggested Working Concentration or Dilution Range:
1:500 - 1:5,000 for immunohisto/cytochemistry
1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates
1:10,000 - 1:200,000 for Western blotting with ECL substrates
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084)
Type:
Antigen: IgG F(c) gamma
Clonality: Polyclonal
Clone:
Conjugation: Peroxidase
Epitope:
Host: Mouse
Isotype: IgG
Reactivity: Human