QuickTiter™ HBsAg ELISA is a sandwich ELISA that is designed for detection and quantitation of Hepatitis B surface antigen in purified virus or unpurified viral supernatant

This assay uses an anti-HBsAg antibody coated plate that binds to any HBsAg present in the sample. A FITC-conjugated anti-HBsAg antibody is then added, which binds to HBsAg sample-antibody complex. This is detected by the addition of an HRP-conjugated anti-FITC antibody.  HBsAg levels in unknown samples are calculated based on the HBsAg standard curve. This kit detects as little as 1 ng/mL of HBsAg.