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Specifications
- Description:CPD Cellular DNA Damage Staining Kit
- Size:96 Assays
- Environmentally Preferable:
- Storage Temperature:4°C
- Cat. No.:102966-234
- Supplier no.:STA-327
Specifications
About this item
OxiSelect™ Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence
Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.