Specifications
- Antibody Type:Primary
- Antigen Name:chemokine (C-C motif) ligand 3
- Antigen Symbol:CCL3
- Clonality:Monoclonal
- Clone:1.2_3E8-2H6-2B6
- Conjugation:Unconjugated
- ELISA:Yes
- Host:Mouse
- Reactivity:Human
- Western Blot:Yes
- Size:0.5 mg
- Neutralisation:Yes
- Form:Lyophilized
- Gene ID:P10147
- Antigen Synonyms:CCL3
- Storage Temperature:Stable at room temperature for at least one month and for greater than a year when kept at –20 °C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. Stable for at least six weeks at 2-4 °C when reconstituted.
- Shipping Temperature:RT
- Immunogen:The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMIP-1α as the immunizing antigen.
- Purification:This IgG1 antibody was purified from ascites fluid by SAS precipitation followed by ion exchange column.
- Cat. No.:10816-214
- Supplier no.:XP-5734-M
Specifications
About this item
Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1alpha (100 ng/mL), a concentration of 5.0-10.0 ug/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 uL/well), a concentration of 2.0-4.0 ug/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1alpha when used with our biotinylated antigen affinity purified anti-human MIP-1alpha (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 ug/mL. Western Blot: To detect hMIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1alpha is 1.0-2.0 ng/lane, under non-reducing conditions.
Type: Primary
Antigen: CCL3
Clonality: Monoclonal
Clone: 1.2_3E8-2H6-2B6
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human