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Anti-CCL7 Mouse Monoclonal Antibody [clone: PEPY042_hMCP3]
Catalog # 10816-212
Supplier:  Prosci
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Anti-CCL7 Mouse Monoclonal Antibody [clone: PEPY042_hMCP3]
Catalog # 10816-212
Supplier:  Prosci
Supplier Number:  XP-5733-M

Specifications

  • Antibody Type:
    Primary
  • Antigen Name:
    chemokine (C-C motif) ligand 7
  • Antigen Symbol:
    CCL7
  • Clonality:
    Monoclonal
  • Clone:
    PEPY042_hMCP3
  • Conjugation:
    Unconjugated
  • ELISA:
    Yes
  • Host:
    Mouse
  • Reactivity:
    Human
  • Western Blot:
    Yes
  • Size:
    0.5 mg
  • Neutralisation:
    Yes
  • Form:
    Lyophilized
  • Gene ID:
    P80098
  • Antigen Synonyms:
    CCL7
  • Storage Temperature:
    Stable at room temperature for at least one month and for greater than a year when kept at –20 °C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. Stable for at least six weeks at 2-4 °C when reconstituted.
  • Shipping Temperature:
    RT
  • Immunogen:
    The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMCP-3 as the immunizing antigen.
  • Purification:
    This IgG1 antibody was purified from ascites fluid on a Protein A affinity column.
  • Cat. No.:
    10816-212
  • Supplier no.:
    XP-5733-M

Specifications

About this item

MCP-3 is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-3 (100ng/mL), a concentration of 1 - 3 ug/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 uL/well), a concentration of 2.0-4.0 ug/mL of this antibody will detect recombinant human MCP-3 when used with our biotinylated antigen affinity purified anti-human MCP-3 (XP-5221Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/mL. Western Blot: To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Type: Primary
Antigen: CCL7
Clonality: Monoclonal
Clone: PEPY042_hMCP3
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human