Specifications
- Antibody Type:Primary
- Antigen Name:tumor necrosis factor
- Antigen Symbol:TNF
- Clonality:Monoclonal
- Clone:2.1_4E10-1H11
- Conjugation:Unconjugated
- ELISA:Yes
- Host:Mouse
- Reactivity:Human
- Western Blot:Yes
- Size:0.5 mg
- Neutralisation:Yes
- Form:Lyophilized
- Gene ID:P01375
- Antigen Synonyms:TNF
- Storage Temperature:Stable at room temperature for at least one month and for greater than a year when kept at –20 °C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. Stable for at least six weeks at 2-4 °C when reconstituted.
- Shipping Temperature:RT
- Immunogen:The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hTNF-alpha as the immunizing antigen.
- Cat. No.:10813-650
- Supplier no.:XP-5711-M
Specifications
About this item
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTNF-alpha (0.5 ng/mL), a concentration of < 0.1 ug/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 uL/well), a concentration of 2.0-4.0 ug/mL of this antibody will detect at least 1000 pg/mL of recombinant human TNF-alpha when used with our biotinylated antigen affinity purified anti-human TNF-alpha (XP-5282Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/mL. Western blot: To detect hTNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-alpha is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Type: Primary
Antigen: TNF
Clonality: Monoclonal
Clone: 2.1_4E10-1H11
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human