Leeds Acinetobacter Medium is a selective and differential medium intended for use as a plating technique to obtain a pure culture of Acinetobacter.

• Selective components inhibit bacterial growth
• Circular, convex, smooth and opaque colonies
• Selective, differential medium

The selective components in Leeds Acinetobacter Medium are traditionally cefsulodin and cephradine, which inhibit the growth of non-Acinetobacter gram-negative bacteria, and vancomycin, which inhibits the growth of gram-positive bacteria

Acinetobacter spp. will produce light pink mucoid colonies with a pink to mauve color diffused into the medium. The colonies are circular, convex, smooth, and opaque with entire margins of 1 to 2 mm in diameter after 24 hours at 35°C.

The presence of fructose and sucrose and neutral red, a pH indicator, allows the differentiation of bacteria based on the products released when the bacteria use fructose and sucrose as a nutrient source. The colonies of those that release acidic products are yellow, while the colonies of those that release alkaline products are red.