The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimized protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.MS-compatible—reagents directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MSSensitive—procedure successfully enriches low abundance proteins (low ng range)Low background—binding, wash, and elution buffers optimized to minimize enrichment of background proteinsRobust—procedure and reagents have been robustly tested with numerous targets to ensure consistent enrichment of low abundance proteins (ng range) with at least 2 peptides identified per proteinThe Pierce MS-Compatible Magnetic IP Kit (Protein A/G) uses high-quality Pierce Protein A/G Magnetic Beads to provide wider flexibility of antibody capture than either Protein A or G alone

The optimized kit components have been formulated to be compatible with downstream LC-MS analysis. After the immunoprecipitation procedure, the target-enriched elution fraction is ready for in-solution tryptic digestion without the need for gel purification, detergent removal, or desalting.This kit has been rigorously validated using numerous target antigens with varying expression levels, including targets previously undetected without enrichment or by western blotting. Additionally, the reagents and procedures have been validated using both manual and automated magnetic separation.Applications:Enrichment of low abundance targets to improve MS sensitivityIdentification of target proteins that are not detectable by western blottingQuantitation of target proteins by LC-MS analysisScreening of antibodies for IP-MSEnrichment of target proteins upstream of targeted analysis (SRM)