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Specifications
- Description:Direct magnetic IP/Co-IP Kit, sufficient for 40 IP reactions using 25 µl of beads
- Size:40 reactions
- Cat. No.:PI88828
- Supplier no.:88828
Specifications
About this item
The direct magnetic IP/Co-IP kit uses NHS-chemistry to covalently immobilize IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation.
- Antibody-free IP-antibody is irreversibly attached to the magnetic beads, resulting in negligible co-elution of intact antibody or heavy and light chains with the purified antigen
- Fast-complete antibody conjugation and immunoprecipitation in less than 4 hours
- Convenient-the IP/co-IP kit contains magnetic beads and all essential buffers for optimal immunoprecipitation
- Antibody compatible-use with any antibody species, class or isotype
- Low nonspecific binding-the bead surface is pre-blocked and all nonreacted NHS-ester groups are fully quenched
- Protocol-compatible-protein coupling to the beads and immunoprecipitation can be performed manually or by automation (e.g., thermo scientific kingFisher instruments)
- Scalable-use only the amount of antibody needed for a single IP experiment or prepare a larger quantity of antibody magnetic beads for multiple experiments
Magnetic IP with NHS chemistry is convenient and allows for the immobilization of IP antibody from any species and subtype without the variations in coupling efficiency seen with Protein A and Protein G. The IP antibody is covalently attached so target proteins or co-IP protein complexes can be eluted and analyzed without antibody contamination. The kit contains an optimized protocol and all buffers and reagents necessary for antibody coupling and high yield IP or co-IP using either manual magnetic stands or automated platforms.
The activated magnetic beads contain N-hydroxysuccinimide (NHS) functional groups, which react with primary amines on antibodies to form stable amide linkages. The antibody is first coupled to the NHS-activated magnetic beads in an amine-free buffer. The antibody-coupled beads are washed to remove any non-covalently bound antibody and quenched. The prepared beads are incubated with the antigen sample, washed to remove non-bound material and eluted in a low-pH elution buffer to dissociate the antigen. The kit includes optimised buffers that minimise non specific binding and maximise antigen yield. Lane marker sample buffer is included for preparing samples for SDS-PAGE analysis. The beads are manually removed from the solution with a magnetic stand or by automation.