Thermo Scientific SulfoLink Immobilization Kit for Proteins is a kit containing all the reagents for preparing the protein sample for efficient immobilization and five columns containing SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

• Specific to sulfhydryl (-SH) groups
• Fast—prepare and couple protein samples in 3.5 hours
• Flexible coupling conditions, use pH 7.5 to 9.0 aqueous buffers, organic solvent or denaturant
• Streamlined protocols for sample preparation, immobilization, and affinity purification
• High capacity—Immobilize 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin

When incubated with a solution of protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the protein to the resin

Applications:
Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification

The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.