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Specifications
- Description:ONPG, powder
- Size:5 g
- Storage Conditions:Upon receipt store at -20˚C protected from moisture and light
- Cat. No.:PI34055
- Supplier no.:34055
Specifications
About this item
Thermo Scientific Pierce ONPG is the preferred colorimetric substrate for ELISA applications involving beta-galactosidase (b-Gal) as the reporter enzyme.
- Product formed is completely soluble
- High extinction coefficient at 405nm
- Substrate yields a yellow product that is easily detectable in the visual range after stopping the reaction with 1M sodium carbonate
When using beta-Galactosidase as the label for proteins in ELISA studies, a wide variety of substrates are available, including: o-Nitrophenyl-beta-D-galactopyranosidase (ONPG), Naphthol-AS-Bl-beta-D-galactopyranosidase (Nap-Gal), and 4-Methyl-umbelliferyl-beta-D-galactopyranosidase (MUm-Gal). However, it is important to choose a substrate with adequate solubility, that uses readily available equipment, and that gives a significant reading over the background. ONPG is a superior beta-Galactosidase substrate option. The product formed is completely soluble and has a high extinction coefficient at 405nm. The substrate yields a yellow product that is easily detectable in the visual range after stopping the reaction with 1M Sodium Carbonate.