This purified calf intestinal alkaline phosphatase (CIP) is supplied in Tris buffer and 50% glycerol for use in protein research methods.

• Purified form, ready to dilute and conjugate without prior dialysis
• Concentrated approximately 20 mg/mL
• High specific activitywhich is typically greater than 1600 units/mg
• Tris buffered solution supplied in 5 mM Tris, 5 mM magnesium chloride and 0.1 mM zinc chloride
• pH ~7.0 in 50% glycerol

The main applications for alkaline phosphatase in molecular biology and protein research are to remove 5'-phosphate groups from DNA or as a reporter system for immunoassays such as ELISA

One unit equals the amount of protein required to hydrolyze one micromole of p-nitrophenyl phosphate (PNPP) per minute at 25°C in a glycine buffer, pH 9.6.

For these latter methods, the enzyme is usually conjugated to specific primary or secondary antibodies and its activity is detected with a color-forming (or light-generating) substrate.