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Specifications
- Description:Co-Immunoprecipitation (Co-IP) Kit
- Size:50 reactions
- Storage Temperature:4 °C
- Cat. No.:PI26149
- Supplier no.:26149
- Sufficient for:50 Reactions using 25 µl of resin
Specifications
About this item
Pierce™ Co-Immunoprecipitation (Co-IP) Kit provides covalent antibody immobilization and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.
- Covalent attachment method and non-reducing elution system
- Covalent antibody immobilization allows prepared IP resin to be reused
- Underivatized agarose beads can be used as negative control for non specific binding
- Co-IP method is compatible with any physiological protein sample buffer
- Perform co-IP experiments using any purified antibody regardless of species or Ig class.
- No dependence on Protein A and Protein G affinity binding
Co-IP is a common approach to study protein:protein interactions that uses an antibody to immunoprecipitate the antigen (bait protein) and co-immunoprecipitate any interacting proteins (prey proteins). This kit covalently couples antibodies onto an amine-reactive resin. This kit is configured to provide the essential tools to effectively perform Co-IP experiments in a manner that yields pure IP and co-IP products free of troublesome IP antibody.