Pierce™ Cypridina Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Cypridina Luc vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca

Naturally-secreting Cypridina luciferase gene, optimised for high expression in mammalian systems
Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimise background by reducing transcriptional read-through
Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells respectively
High-copy pUC bacterial DNA replication origin

pMCS-Cypridina Luc vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Cypridina luciferase reporter.
pCMV-Cypridina vector has the luciferase gene under the CMV (Cytomegalovirus) promoter. Strong (CMV) constitutive promoters for co-transfection and normalisation.
pTK-Cypridina vector has the luciferase gene under the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter. Weak (TK) constitutive promoters for co-transfection and normalization.

The gene encodes a naturally-secreted bioluminescent Cypridina luciferase (62 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

: For Research Use Only. Not for use in diagnostic procedures.

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