Immunoassay buffer is used as a 1X in all immunoassays (immunofluorescence, IHC, ELISA, WB etc.) as a washing buffer and it can be used as a dilution buffer for antibodies; however it does not contain any carrier proteins.
- Buffer also does not contain any phosphate, sodium azide or mercury as preservatives
- Can be used for dilution of all antibodies, including peroxidase, antibodies to phosphoproteins
- Not suitable for dilution of antibodies to S100 proteins
Application: Dilute this 10X buffer 10 times with DI water. (90 ml water + 10 ml this buffer – mixed well). Wash 3-5 times with this buffer in between the steps of your immunoassay protocol.
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