A very stable buffer, used with Osmium tetroxide; provides excellent fixation, better stability and buffering capacity.
The pH can be adjusted by varying the amount of hydrochloric acid in the final volume of 200 ml.
Preparation Instructions: Pour 9 ml of 2 N HCl; add 5.34 ml of Sym-Collidine, mix well and add distilled water up to 200 ml for a pH 7.4 solution (Bennett and Luft., Biophys and Biochem. Cytol., 6, 113, 1959).
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