Human Recombinant NGF beta (from E. coli)

About this item

β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. The functional form of Recombinant Human β-NGF is a non-covalently-linked homodimer of two 13.5 kDa, polypeptide monomers that each contain 120 amino acids and three disulfide bonds, which are required for biological activity.Manufactured using all Animal-Free reagents.

Carrier free
Animal-free

SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR

Verified by N-terminal and Mass Spectrometry analyses (when applicable).

Ordering information: For research use only. Not for use in diagnostic or therapeutic procedures.

Specifications

Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
−20 °C
Endotoxin Content:
<0.1 ng/μg of protein (<1 EU/μg).
Biological Activity:
Assay 1: Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED₅₀ is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁶ units/mg.
Assay 2: Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The expected ED₅₀ is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁶ units/mg.
Animal-Free:
Y
Protein/Peptide Name:
NGF beta
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Formulation:
Lyophilized
Shipping Temperature:
RT